CloneID: PVSS21E
Antigen Long Description: The original antibody was raised by immunizing a dromedary with poliovirus type 1 Sabin strain.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a chimeric antibody, developed from the original camelid VHH antibody, specifically engineered for enhanced compatibility with existing reagents, assays, and techniques.
Uniprot Accession No.: P03300
Specificity Statement: This antibody is specific for Capsid proteins VP1 (GH loop, EF loop and C beta strand) and VP2 (EF loop) of the polio genome polyprotein.
Application Notes (Clone): The binding strength of the original antibody to poliovirus antigens was determined using surface plasmon resonance (Thys et al., 2011; PMID:21371506). The original antibody was used for a neutralization assay against three types of poliovirus. This antibody showed strong neutralization in type 1 polioviruses. The cytotoxicity of this antibody on HeLa cells was also evaluated. It showed no cytotoxicity (Thys et al., 2010; PMID:20566349). The effect of this antibody on virus entry and uncoating was determined. It showed change in the behavior of the polio particles (Schotte et al., 2014; PMID:24501405). The structure of this antibody was determined using cryo-EM (Strauss et al., 2016; PMID:26764003).
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