CloneID: N20 (3C12)
Antigen Long Description: The original antibody was prepared by immunizing BALB/c mice with attenuated Gintexin antigen purified from the crude venom of Gloydius intermedius.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: A0A096XPP4
Specificity Statement: This antibody binds the gintexin A type PLA2 protein found in the venom of Gloydius intermedius. Gintexin A is Asn-49 type PLA2 isomer that has no enzymatic activity and neurotoxicity. Gentexin is a heterodimer comprising gintexin A and gentexin B. It is estimated that, after the venom enter the blood system GintexinA acts as a "director" to guide the neurotoxin heterodimer to bind to the membrane receptor at the phrenic nerve NMJ synapse, and then Gintexin B exerts neurotoxicity (blocks the diaphragm NMJ synaptic transmission and stops breathing).
Application Notes (Clone): This antibody was capable of recognizing attenuated gintexin A of Gloydius intermedius and a fusion protein containing gintexin A in a western blot assay. The binding affinity of this antibody for gintexin A was determined using ELISA. 100 μg this antibody can neutralize at least 2×LD50 of Agkistrodon venom. In vivo, the neutralizing activity of this antibody is reported to be about 21.1 LD50/mg. The sensitivity of HRP-N20 antibody for immunospot detection of Agkistrodon intermedius venom is 12.5 ng/mL.
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