CloneID: Mab-1 (23A8-1)
Antigen Long Description: The original antibody was generated by immunizing mice with bacterially produced RVFV Gn protein.
Buffer Composition: PBS only.
Uniprot Accession No.: P21401
Specificity Statement: The antibody is specific for the Gn protein binding a linear epitope in the B structural domain (aa 366 to 440). Gn is a surface glycoprotein of RVFV.
Application Notes (Clone): The specificity of the original format of the antibody to Gn from RVFV lysate was confirmed by ELISA analysis (EC = 3.9 ng/mL). The antibody was potently neutralizing, with an IC50 of 28 ng/ml. To determine the protective potential of the IgG1 format of the antibody, it was administrated to C57BL/6 mice in a RVFV lethal challenge model. The antibody was able to protect the mice, with only one mouse succumbing to disease. The IgG2a version of the antibody was constructed. It exhibited an RVFV neutralization capacity and ELISA affinity similar to that of its IgG1 counterpart. Further, the IgG2a version showed significantly higher NK cell degranulation. In vivo experiments demonstrated that the IgG2a format of the antibody fully protected mice from the lethal challenge (Cartwright et al., 2021; PMID:34494884).
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