CloneID: Mab-2 (23B5-1)
Antigen Long Description: The original antibody was generated by immunizing mice with bacterially produced RVFV Gn protein.
Buffer Composition: PBS only.
Uniprot Accession No.: P21401
Specificity Statement: The antibody is specific for the Gn protein binding an epitope in the β1 structural domain (aa 301 to 365). Gn is a surface glycoprotein of RVFV.
Application Notes (Clone): The specificity of the original format of the antibody to Gn from RVFV lysate was confirmed by ELISA analysis (EC = 8.8 ng/mL). The antibody was neutralizing, with an IC50 of 1.5 ng/ml. To determine the protective potential of the IgG1 antibody, it was administrated to C57BL/6 mice in a RVFV lethal challenge model. IgG1 isotype control-treated mice succumbed to disease within 3 days of challenge, while the antibody protected mice, with only one mouse succumbing to disease. The IgG2a version of the antibody was constructed. It exhibited an RVFV neutralization capacity and ELISA affinity similar to that of its IgG1 counterpart. Further, the IgG2a version showed significantly higher NK cell degranulation. In vivo experiments demonstrated that the IgG2a format of the antibody fully protected mice from lethal challenge (Cartwright et al., 2021; PMID:34494884).
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