CloneID: Mab-3 (32A8-1)
Antigen Long Description: The original antibody was generated by immunizing mice with bacterially produced RVFV Gn protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P21401
Specificity Statement: The antibody is specific for the Gn protein binding a linear epitope in the A structural domain (aa 154 to 300). Gn is a surface glycoprotein of RVFV.
Application Notes (Clone): The specificity of the original format of the antibody to Gn from RVFV lysate was confirmed by ELISA analysis (EC = 23.0 ng/mL). The antibody was neutralizing, with an IC50 of 12.2 ng/ml. The IgG2a version of the antibody was constructed. It exhibited an RVFV neutralization capacity and ELISA affinity similar to that of its IgG1 counterpart. Further, the IgG2a version showed significantly higher NK cell degranulation. In vivo experiments demonstrated that the IgG2a format of the antibody fully protected mice from lethal challenge (Cartwright et al., 2021; PMID:34494884).
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