CloneID: 3JA18
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated from naive human single-fold scFv libraries by panning against the recombinant HA2 ectodomain protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length reformatted human antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P03454
Specificity Statement: The antibody specifically binds the stem region of HA. The antibody recognises influenza H1N1, H3N2, H5N1, H5N2 and H7N9.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant HA2 by western blot analysis. Surface-enhanced Raman spectroscopy assay showed the antibody has high affinity for rHA2 and virus particles. A neutralization assay with H5pp was carried out to evaluate the inhibition of virus entry by the antibody. The results showed that the original format of the antibody can significantly reduce H5pp-mediated infection. Further, an influenza virus PRNT assay was performed on the antibody using H1N1 virus. The antibody neutralized H1N1 virus and significantly decreased the number of plaques arising from lysis of host cells (MDCK) (Li et al. 2016; PMID: 26446888).
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