CloneID: 19D9D6
Antigen Long Description: The original antibody was generated by immunizing BALB/c JYco mice with a truncated recombinant protein corresponding to the immunodominant region (residues 1–120) of the Hepatitis C Virus (HCV) purified nucleocapsid protein (HCV C120-GST fusion protein).
Origin Pub PMID: 12574359
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for the protein sequence QIVGGVYLL located at residues 29-37 of the HCV core protein. Hepatitis C virus (HCV) is the primary cause of hepatitis transmitted through blood transfusion. It is an enveloped virus that contains a single-stranded, positive-sense RNA genome of approximately 9600 nucleosides. This genome encodes a polyprotein of 3011 amino acids, which undergoes post-translational cleavage to form both structural and nonstructural proteins. Among the structural proteins, the core protein is derived from the amino terminus of the viral polypeptide (amino acids 1-191).
Application Notes (Clone): This antibody was used for an indirect ELISA to detect anti-HCV core response in human sera, an ELISA competition assays to assess the interaction between monoclonal antibodies and anticore human sera, a sandwich ELISA for the capture and detection of viral core antigen in chronic HCV patient sera, and a sandwich ELISA for the detection of recombinant core protein (Jolivet-Reynaud et al., 1998; PMID: 9829633). The crystal structure of the Fab version of this antibody was solved in complex with an HCV core protein-derived peptide (residues 13-40), revealing the hydrophobic nature of the recognized epitope (Ménez et al., 2003; PMID: 12574359).
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