CloneID: 1F5
Antigen Long Description: The original antibody was generated by immunizing mice with recombinant soluble constructs obtained from Nipah or Hendra virus.
Buffer Composition: PBS only.
Uniprot Accession No.: Q9IH63; O89342
Specificity Statement: This antibody binds a pre-fusion conformational epitope on the F glycoprotein of the Nipah and Hendra virus. The Nipah and Hendra virus are spread by bats.
Application Notes (Clone): This antibody is reported to broadly neutralize Nipah and Hendra virus by blocking membrane fusion. The cryogenic-electron microscopy (cryo-EM) structures of the antibody in complex with HeV F was determined. Biolayer interferometry was used to characterize the binding of the 1F5 Fab fragments to prefusion NiV F or HeV F ectodomain trimers immobilized on the surface of biosensors. The equilibrium dissociation constants (Kd) of 1.47 and 1.24 nM for attachment of Fab fragments of 1F5 to NiV F or HeV F, respectively. To further characterize this antibody, a kinetic assay was performed on HeV F- or NiV F- or NiV F S69A using the mouse version of this antibody. To analyze the ability of this antibody to prevent and cure disease, a fusion inhibition assay was performed on CHO745 cells transfected with HNV F and G glycoproteins using the mouse IgG version of this antibody. Further a plaque reduction assay was performed NiV-M, NiV-B or HeV using both the human and mouse IgG versions of this antibody. The humanized version of the antibody broadly inhibited NiV-M, NiV-B and HeV with IC50 ranging between 0.2 and 1.3 µg/ml (Dang et al., 2021; PMID:33927387).
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