CloneID: t1E4
Antigen Long Description: The original antibody was generated by immunizing MRL/lpr mice with tcK2SP(Xa).
Buffer Composition: PBS only.
Uniprot Accession No.: P14210
Specificity Statement: The antibody is specific for HGF. It can bind to the two chains and to the single chain HGF. It recognizes an epitope located within the K4-SP region.
Application Notes (Clone): The specificity of the original format of the antibody for HGF was confirmed by ELISA analysis. The authors investigated if the antibody has any effect on the HGF-Met signaling. Met activation was strongly inhibited by the antibody, with an IC50 value of ~0.15 μg/ml or 0.1nM. In particular, antibody caused dose dependent reduction of the HGF-induced phosphorylation levels of Met (Tyr1234/1235), Akt (Ser473), and ERK (Thr202 and Tyr204). The inhibitory action of the antibody was further evaluated by two cell-based assays. The effect of all antibody on the HGF-induced scattering of Madin-Darby canine kidney (MDCK) cells was evaluated, and confirmed that only it could strongly suppress the scattering behavior of the cells when added at 10 μg/ml. Furthermore, the antibody was able to inhibit the HGF-stimulated migration of human liver bile duct carcinoma cells with a similar concentration dependency as the inhibition of Met phosphorylation in EHMES-1 cells. The antibody immunoprecipitated both scHGF and tcHGF. The binding affinity of the antibody toward the HGF proteins was tested by SPR.
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