CloneID: 19D12
Antigen Long Description: The original antibody was generated by immunizing Xenomouse™ IgG2κλ and IgG4κλ with KLH-conjugated human hepcidin peptide 'DTHFPICIFCCGCCHRSKCGMCCKT'.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG2 format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P81172
Specificity Statement: This antibody is specific for amino acids 'DTHFPICIFCCGCCHRSKCGMCCKT' of human hepcidin-25 and does not cross react with murine hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide stabilized by 4 disulfide bonds that plays a major role in innate immunity and iron homeostasis. It controls the delivery of iron to blood plasma from intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and from iron-storing hepatocytes. Hepcidin acts by binding to and inactivating the sole cellular iron exporter, ferroprotein, which delivers iron to plasma from all iron-transporting cells.
Application Notes (Clone): The original IgG antibody binds human hepcidin-25 with a binding affinity of Kd= 340 pM (PMID: 23945155). This antibody can be used in combination with another anti-hepcidin antibody 19D12 for detection of human hepcidin in human serum using a sandwich ELISA. 19D12 was used as a capture antibody and 23F11 was used for detection. The limit of sensitivity for the assay was 30 pg/mL (PMID: 23945155). This antibody was also used for immunohistochemical analysis of formalin-fixed, paraffin-embedded sections of prostrate cancer tissues (PMID: 25858146). This antibody was also used to block hepcidin in a study to confirm the functionality of hepcidin in breast tumor spheroids. As expected, blockade of hepcidin increased ferroprotein, indicating that hepcidin synthesized by these cells functions in an autocrine fashion and exerts its expected biological activity (PMID: 29695834).
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