CloneID: 3A3
Antigen Long Description: The original antibody was generated by immunizing rabbits with Her-2.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Rabbit IgG format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P04626
Specificity Statement: The antibody is specific for Her-2.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis (affinity constant = 1.09 × 10^11M^-1). The antibody specifically detected HER-2 protein in Western blot analysis. The antibody 3A3 and the commercial antibody EP3 were used to stain breast cancer tissue. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 3A3 was higher than the commercial antibody. The antibody and the commercially available antibody EP3 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113621069A).
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