CloneID: 9C12
Antigen Long Description: The original antibody was generated by immunizing mice with Ad type 5 (Ad5).
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P04133
Specificity Statement: The antibody binds hexon protein from Ad5 particles. In particular, it binds to HVRs 2, 5 and 7 of the hexon. Hexon is the major coat protein of adenovirus.
Application Notes (Clone): The antibody was able to neutralize Ad5. Cryo-EM reconstruction of the Ad5-9C12 complex showed that the capsid is coated by a meshwork of bivalently bound antibody molecules. The antibody bound to the hexon protein of Ad5 particles denatured as shown by western blot analysis (Varghese et al., 2004; PMID: 15507619).The crystal structure of scFv fragment of the antibody in complex with human adenovirus C5 hexon was determined (Schmid et al., 2018; PMID: 29386504). The specificity of the original format of the antibody was confirmed by ELISA analysis (Moyer et al., 2016; PMID: 26491163). Wild-type viruses were immunoprecipitated using the antibody (Myerset al., 2013; PMID: 23487468). The binding affinity of the Fab fragment to Adv5 hexon was measured by surface plasmon resonance (Kd= 1 nM) (Bottermann et al., 2016; PMID: 27881870). The antibody detected hexon on A549 cells by immunofluorescence analysis (Smith et al., 2008; PMID: 18448546).
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