CloneID: 1F7
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with pooled human polyclonal HIVIG.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The anti-idiotypic antibody recognizes antibodies that react to different components of the HIV virus, such as gp120, p24. The antibody does not react with antibodies derived from SLE and B cell lymphoma.
Application Notes (Clone): The HIV-1 antigen capture ELISA was used to test the binding of human monoclonal anti-HIV-1 to the antibody. The antibody bound to anti-p24 antibodies and anti-gp120 antibodies. Western blot analysis confirmed that the antibody reacts with human antibodies of different HIV-1 antigen specificities (Wang et al., 1992; PMID: 1378015). SHIV-infected macaques were immunized with the antibody. Significant increases in breadth and potency of HIV-1 neutralizing antibody titers to one or more virus strains were detected in all three of the 1F7 immunized macaques (US6057421A).
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