CloneID: mem-G/9
Antigen Long Description: The original antibody was generated by immunizing mice with native, properly folded, beta-2-microglubulin (b2m)-associated HLA-G.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P17693
Specificity Statement: This antibody binds an epitope in the alpha 1 domain of human HLA-G. It binds the native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. It is also reported to cross react with HLA-G3.
Application Notes (Clone): This antibody is not suitable for detecting denatured human HLA-G protein in a western blot. This antibody can be used for the detection of soluble HLA-G in a sample using ELISA (PMID: 10902607). This antibody can effectively detect HLA-G and HLA-G3 on K653-HLA-G1, K562-G3 transductants, M8-HLA-G1 and JEG-3 cells in a flow cytometric analysis (PMID: 12590976; 22738368). This antibody was also used for studying the expression of HLA-G on the surface of JEG-3 cells using immunofluorescence staining (PMID: 22738368). This antibody can be used for immunohistochemical analysis of cryopreserved serial sections of trophoblast tissue. This antibody is not suitable for immunohistochemical staining of paraffin sections (PMID: 12590976). This antibody was also reported to be used in isolation of HLA-G+ cells for prenatal screening (PMID: 35498932).
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