CloneID: G8
Heavy Chain modification: His-Tagged
Antigen Long Description: This antibody was generated by panning a phage displayed repertoire derived from the immunoglobulin genes of the chicken against HSP65.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P0A521
Specificity Statement: This antibody is specific for the HSP65 protein from Mycobacterium bovis. Together with its co-chaperonin GroES, HSP65 plays an essential role in assisting protein folding. Mycobacterium bovis is the causative agent of tuberculosis in cattle.
Application Notes (Clone): An ELISA was performed using the scFv version of this antibody on HSP65 to determine how efficiently the antibody can bind the antigen. Furthermore, an immunoblot was performed on HSP65 using this antibody (Wemmer et al, 2010; pmid:20299243).
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