Anti-ICOS ligand (Clone 325)

Anti-ICOS ligand [Clone 325], Recombinant, IgG1 lambda, Human

CloneID: Clone 325

Antigen Long Description: The original antibody was isolated from a human naïve scFv phage library and panning against B7RP-1-Fc protein.

Buffer Composition: PBS only.

Uniprot Accession No.: O75144

Specificity Statement: This antibody binds human B7RP-1, which is a ligand for T-cell-specific cell surface receptor ICOS. It acts as a costimulatory signal for T-cell proliferation and cytokine secretion and also induces B-cell proliferation and differentiation into plasma cells.

Application Notes (Clone): The scFv version of this antibody binds B7RP-1-Fc with a Kd= 22nM. The binding specificity of this antibody was confirmed using ELISA. This scFv version of this antibody was shown to bind B7RP-1-Fc in an ELISA. This antibody recognized B7RP-1 molecules expressed on B cells, as assessed by flow cytometry. This antibody is capable of inhibiting interaction between ICOS-Fc and B7RP-1-Fc. This antibody also exhibited inhibitory activity in a proliferation assay of T cells stimulated with anti-CD3 mAb and B7RP-1-Fc (Maeda et al., 2009; PMID: 20065650).

• SKU: ABAAb03803-10.0-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03803-10.0-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Recombinant
• Application: ELISA, Flow Cytometry, Blocking, Inhibition
• Isotype: IgG1 lambda
• Host: Human