CloneID: MsMab-1
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing BDF1 mice with synthetic IDH1-R132G peptide of 19 amino acids (GGVKPIIIGGHAYGDQYRA) conjugated with KLH.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: O75874; P48735
Specificity Statement: This antibody binds human isocitrate dehydrogenase 1 (IDH1) having R132H, R132C, R132S, R132G and R132L mutations and does not cross react with wild-type IDH1 protein. It also recognizes IDH2-R172M (GGTKPITIGMHAHGDQYKA) and IDH2-R172S (GGTKPITIGSHAHGDQYKA). IDH1 is an enzyme that catalyzes the NADP+-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. It plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. The NADPH produced from isocitrate dehydrogenase 1 is involved in the breakdown of fats for energy, and it also protects cells from potentially harmful molecules called reactive oxygen species. Mutation in IDH1 are found in several genetic conditions and in some types of cancer, including gliomas, cartilaginous tumors, acute myelogenous leukemia, myelodysplastic syndromes, and brain cancer.
Application Notes (Clone): The binding reactivity of this antibody to wild-type IDH1 and its mutants (R132H, R132C, R132S, R132G and R132L) peptides was determined using ELISA. Western blot analysis also revealed that MsMab-1 reacted with recombinant proteins of IDH1-R132H, IDH1-R132S, and IDH1-R132G, but not with wild type IDH1 and other IDH1 mutations. This antibody also recognizes IDH2-R172M protein, despite that the IDH1-R132G peptide shows only 73.7% identity with the equivalent portion of IDH2-R172M (GGTKPITIGMHAHGDQYKA). This antibody successfully stained the IDH1-R132S or IDH1-R132G-expressing glioma cells in immunohistochemistry (PMID: 23782684). This antibody can bind IDH2-R172S mutant of IDH2 in an ELISA and can recognize PA-tagged IDH2-R172S recombinant protein in a western blot. This antibody was also used to stain IDH2-R172S-expressing osteosarcoma tissues in immunohistochemistry (PMID: 24403254). A complete summary of the reactivity of this antibody towards various IDH1 and IDH2 mutants can be found in Liu et. al, 2013 (PMID: 24403254).
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