CloneID: 9H10
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing IL-1α/β double KO mice with mouse IL-1 alpha.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P01582
Specificity Statement: This antibody binds mouse Interleukin-1 alpha, which is a cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells.
Application Notes (Clone): The original mouse IgG antibody binds mouse IL-1A with a binding affinity of Kd= 0.17 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 2.00E−10M. This antibody was used in combination with 10G11 (anti- mouse IL-1B) antibody for the generation of anti-mouse IL-1α/β DVD-Ig molecule (US7612181).
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