Anti-IL-3Ra (57A07)

Anti-IL-3Ra [57A07], Recombinant, IgG1-Fc Fusion, Human

CloneID: 57A07

Buffer Composition: PBS with 0.02% Proclin 300.

Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P26951

Specificity Statement: This antibody is specific for the alpha subunit of IL-3R

Application Notes (Clone): The binding of the original version of this antibody (VHH) to IL3-Ra-expressing viral lipoparticles was characterized in an ELISA (K_D 0.783 nM). In a FACS binding assay, this antibody exhibited an EC₅₀ of 0.66 nM, 1.3 nM, and 0.24 nM for MOLM-13, THP-1, and HEK-IL-R3a, respectively, and an IC₅₀ of 1.20 nM in competition against clone 7G3. In its bi-specific constructions with clones 14D09 and 281F12, it served as an anchor (US20220242958).

• SKU: ABAAb04370-10.159
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab04370-10.159
• Package Unit: 100 μg
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Recombinant
• Application: ELISA, Fluorescence-Activated Cell Sorting (FACS)
• Isotype: IgG1-Fc Fusion
• Host: Human