CloneID: scFvs 104
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was generated by immunizing rabbits from the New Zealand white strain with HIV-1 IN protein developing a combinatorial single-chain variable fragment (scFv) library against IN. The antibody was isolated by panning against immobilized HIV-1 IN protein.
Buffer Composition: PBS only.
Uniprot Accession No.: P04585
Specificity Statement: The antibody is specific for the catalytic and C-terminal domains of IN protein. The antibody does not bind to HIV-1 PR protein. This enzyme catalyses the integration of the HIV genome into the chromosome of the host cell. The HIV-1 IN consists of a 288 amino-acid (aa) protein (32 kDa) encoded at the 3′-end of the HIV pol gene and it contains three distinct domains: N-terminal, catalytic core, and the C-terminal.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected the HIV-1 IN protein by western blot analysis. The scFv fragment inhibited the activity of HIV-1 IN in vitro. HIV-1 replication was inhibited by the scFv localized in cytoplasm and nucleus of HeLa cells. Further, the scFv fragment specifically inhibited HIV-1 DNA integration in Hela-P4 cells. Jurkat cell lines stably expressing scFv intrabody were employed to determine whether the intracellular expression of the scFv fragment was able to prevent HIV-1 replication. Jurkat–scFv fragment cell lines showed approximately 60%–80% inhibition of HIV-1 p24 antigen production compared with the parental Jurkat cells (da Silva et al., 2012; PMID: 23586912).
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