CloneID: 339.1
Antigen Long Description: The original antibody was raised by immunizing a Female Sprague-Dawley rat with alpha-5 beta-1 purified from mouse placenta or with a mouse alpha-5 beta-1-Fc fusion protein
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P11688; P09055
Specificity Statement: This antibody is specific for alpha-5 beta-1
Application Notes (Clone): The original antibody was used for an ELISA on alpha-5 beta-1-Fc fusion protein. The original antibody was used for surface plasmon resonance on alpha-5 beta-1-Fc. It showed a binding affinity of 0.59 nM. This antibody was used for an Annexin V cell death assay. Furthermore this antibody was used for immunohistochemistry on Xenograft tumours derived from cells inoculated subcutaneously in ICR-SCID or SCID-Beige mice (Bhaskar et al., 2007; PMID:18042290). This antibody was used to test the effect of alpha-5 and beta-1 integrin blockers on OvCa cell lines HeyA8 and SKOV3ip1. Further this antibody was used for in vivo testing of alpha-5 and beta-1 integrin blocking antibodies using the HeyA8 xenograft model of OvCa metastasis. It showed a decrease in the number of tumours in the subjects (Kenny et al., 2014; PMID:25202979).
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