CloneID: Ab835
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG4 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P18627
Specificity Statement: The antibody is specific for human LAG-3. The antibody does not cross react with cynomolgus LAG-3.
Application Notes (Clone): The antibody is a humanized version of the mouse antibody mab7c. The specificity of the original format of the antibody was confirmed by ELISA analysis (EC50=0.12 nM). The binding activity of the humanized antibody was tested using the hLAG-3 positive cell line, and the results showed that the antibody was positive for hLAG-3. The binding activity of the antibody and hLAG-3 positive cells was EC50 = 0.41 nM. The binding affinity of the antibody to hLAG-3 was measured by surface plasmon resonance (Kd= 0.52 nM). The antibody blocked the binding of MHCII and human LAG-3 with IC50 = 2.27 nM and it prevented LAG-3 and MHCII from binding to activate human T cell activity (release IL2) (EC50=1.74ug/ml) (CN110172099).
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