Anti-LAG-3 (ADI-31798)

Anti-LAG-3 [ADI-31798], Recombinant, IgG1 kappa, Mouse

CloneID: ADI-31798

Antigen Long Description: The original antibody was isolated from a yeast display library by two rounds of screening against human LAG-3.

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.

Uniprot Accession No.: P18627

Specificity Statement: This antibody binds human LAG-3. The human LAG-3 is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor on activated T-cells.

Application Notes (Clone): This antibody can bind human LAG-3 in an ELISA. This antibody binds human LAG-3 with an affinity of Kd= 9.73 X 10^(-10) nM. The binding specificity of this antibody to human LAG-3 expressed on the surface of HEK293 and activated CD4+ T cells was done using flow cytometry. This antibody binds hLAG-3 expressed on the surface of HEK293 cells with an EC50 of 0.201 nM and activated CD4+ T cells with an EC50 of 0.00595 nM. This antibody can also effectively block the binding of LAG-3 and its ligand MHCII (HLA-DR) with an IC50 of 4.701 nM. The tumor suppressive effects of this antibody was studied in A375 human PBMC model (CN112166125).

• SKU: ABAAb03439-1.1-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03439-1.1-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Recombinant
• Application: ELISA, Flow Cytometry
• Isotype: IgG1 kappa
• Host: Mouse