CloneID: BJRbL03
Heavy Chain modification: His-Tagged
Antigen Long Description: This antibody was generated by immunizing a rabbit with Mycobacterium tuberculosis H37Rv cell wall.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for the LAM protein of Mycobacterium tuberculosis but shows cross-reactivity with other Mycobacterium species like M. bovis. M. tuberculosis causes the disease tuberculosis.
Application Notes (Clone): The ability of the rabbit version of this antibody to bind LAM was determined using ELISA on LAM. Furthermore, the affinity of this antibody to LAM was determined using a ForteBio Octe machine. Finally, the ability of this antibody to distinguish isolates of M. tuberculosis (MTB), M. avium-intracellulare (MAI), M. abscessus, and M. fortuitum-abscessus (MA-MF) was assessed using flow cytometry; it bound all bacteria (Yan et al., 2021; PMID: 32146163). This antibody was used as a capture antibody in a sandwich ELISA (Yan et al., 2023; PMID: 37764103).
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