Anti-M-CSF (Celine)

Anti-M-CSF [Celine], Recombinant, IgG1 lambda, Human

CloneID: Celine

Antigen Long Description: The original antibody was selected from a commercially available phage display library. The library was panned against the receptor-binding domain of human M-CSF (rbdM-CSF).

Buffer Composition: PBS with 0.02% Proclin 300.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P09603

Specificity Statement: This antibody is specific for human M-CSF. It has been shown to cross-react with cynomolgus monkey M-CSF. M-CSF is a secreted cytokine that influences hematopoietic stem cells to differentiate into macrophages or other related cell types.

Application Notes (Clone): The original version of this antibody (human IgG1) was tested for its specificity for human M-CSF and cross-reactivity for cynomolgus M-CSF using ELISA. This antibody binds M-CSF expressed on cells, as shown in a FACS assay that exhibited an EC₅₀ value of 9.7 nM and 13 nM against M-CSF-expressing transfected CHO cells and MDA-MB 231 cells, respectively. This antibody successfully inhibited human and cynomolgus M-CSF binding to recombinant human M-CSF receptor with an IC₅₀ of 4.7 pM and 6.9 pM, respectively. This antibody successfully inhibited the proliferation of M-NFS-60 cells induced by recombinant and native human and cynomolgus M-CSF with an IC₅₀ of 2.7 pM and 23.8 pM for recombinant and native human M-CSF, respectively, and 3.0 pM and 72.4 pM for recombinant and native cynomolgus M-CSF, respectively (US10081675B2).

• SKU: ABAAb04519-10.0
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab04519-10.0
• Package Unit: 100 μg
• Quantity Unit: STK
• Reactivity: Human, Cynomolgus Monkey (Primate)
• Clonality: Recombinant
• Application: ELISA, Inhibition, Fluorescence-Activated Cell Sorting (FACS)
• Isotype: IgG1 lambda
• Host: Human