CloneID: H16.1A
Heavy Chain modification: His-Tagged
Antigen Long Description: The original antibody was raised by immunizing a BALB/c mouse with HPV-16 or HPV-18 L1 VLPs.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P03101
Specificity Statement: This antibody is specific for a conformational epitope covering amino acids 266–297 and 339–365.
Application Notes (Clone): The fab version of this antibody was used a neutralization assay, in short both a pre- and post-virus attachment assay was performed on 293TT cells with pseudo virus. The structure of this antibody was determined using cryo-EM (Guan et al., 2015; PMID:25996608). This antibody was used for surface plasmon resonance on virus like particles (Towne et al., 2013; PMID:23159495). This antibody was used for an ELISA on HPV-16 L1wt VLPs. This antibody was used for a neutralization assay on HPV-16 pseudo virions, in short the antibody was incubated with HPV-16 pseudo virions, then an attempt was made to infect cervical carcinoma C33A cells with the mixture. This antibody showed some neutralization (Christensen et al., 2001; PMID:11878901).
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