Anti-mCherry (LAM-4)

Anti-mCherry [LAM-4], Recombinant, IgG-Fc Fusion, Rabbit

CloneID: LAM-4

Antigen Long Description: The original antibody was generated by immunization of Llama glama with mCherry fluorescent protein. A VHH cDNA library was generated by high-throughput DNA sequencing of a marrow lymphocyte from an immunized llama. Later on this was combined with mass spectrometric (MS) identification of high affinity VHH regions derived from serum of the same animal.

Buffer Composition: PBS only.

Uniprot Accession No.: Q5S3G8; X5DSL3

Specificity Statement: This antibody binds mCherry and can also bind DsRed.

Application Notes (Clone): This antibody can be used to detect mCherry fluorescent protein and DsRed in an ELISA. This antibody binds immobilized mCherry with a binding affinity of Kd= 0.18 nM. The Alexa Fluor® 488 conjugated version of this antibody was used to immunofluorescently stain S. cerevisiae expressing mCherry-tagged histone H2B (PMID: 25362362). This antibody was used for the visualization and manipulation of red fluorescent labelled proteins in live cells by using nanobodies targeting fluorescent protein tags (PMID: 31122229).

• SKU: ABAAb03476-23.159-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03476-23.159-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Various species
• Clonality: Recombinant
• Application: Immunofluorescence, ELISA, Immunohistochemistry
• Isotype: IgG-Fc Fusion
• Host: Rabbit