CloneID: 04CT2.2.3
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with MMAE-cysteine-KLH conjugate in equal volume of complete Freund's adjuvant.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a chimeric rabbit IgG antibody made using the variable domain sequences of the original mouse IgG1 format for improved compatibility with existing reagents, assays, and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody binds auristatin (MMAE, MMAF) which is a cytotoxic drug (payload) employed in the ADC field. Monomethyl auristatin E (MMAE, vedotin) is a very potent antimitotic agent that inhibits cell division by blocking the polymerization of tubulin. The family of auristatins are synthetic analogues of the antineoplastic natural product Dolastatin 10, ultrapotent cytotoxic microtubule inhibitors that are clinically used as payloads in antibody-drug conjugates. As an antibody-drug conjugate or ADC, MMAE is linked to a monoclonal antibody (mAb) that recognizes a specific marker expression in cancer cells and directs MMAE to a specific, targeted cancer cell.
Application Notes (Clone): The binding activity of this antibody towards MMAE-BSA conjugate was determined using ELISA. This antibody is also capable of binding to MMAE-conjugated antibody drugs and can be used for their detection in cancer research (CN114181307).
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