CloneID: 2-12M1
Antigen Long Description: The original antibody was raised by immunizing BALB/c mice with preparations of M1 antigens isolated from ovarian mucinous fluid of a pure endocervical type from an ALe(a-b+) patient.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P98088
Specificity Statement: This antibody is specific for the C-terminus of MUC-5AC.
Application Notes (Clone): The original version of this antibody was used for a western blot on different recombinant versions of the MUC-5AC protein (Lidell et al., 2008; PMID:18167142), on mucin proteins obtained from whole human stomachs (Mall et al., 1999; PMID:10343135), and on mucin proteins (Bara et al., 1988; PMID:2460087). This antibody was also used to stain MUC5AC in immunofluorescence on COS-7 cells transfected with pSecTag2 carrying fragments of the 59 end cDNA of MUC5AC (Nollet et al., 2004; PMID:15165482), and on BxPC-3, HT-29, LS174T, MCF-7, Calu-3 and Capan-1 cells (Liu et al., 2015; PMID:25595893). Furthermore this antibody was also used for immunoradiometric staining on fractions obtained during protein purification (Bara et al., 1991; PMID:1988372), and on a colon mucin -preparation of an 0 Le(a-b-) patient (Bara et al., 1988; PMID:2460087). Additionally this antibody was used for an ELISA on a M1 antigen preparation. And finally, this antibody was used for immunohistochemistry to stain the M1 antigen in fetal colonic mucosae (Bara et al., 1986; PMID:3524800).
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