CloneID: SurfS2.2
Antigen Long Description: This antibody was generated by panning a phage library generated from sheep were infected via the oral route with two doses of 109 CFU of a low-passage M. avium subsp. paratuberculosis strain W. against mycobacterial cell suspensions were harvested from culture.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for Mycobacterium avium. Mycobacterium avium together with Mycobacterium intracellulare causes the disease called Mycobacterium avium-intracellulare infection or Mycobacterium avium complex infection in humans.
Application Notes (Clone): The scFv version of this antibody was immunoblotted on M. avium subsp. paratuberculosis lysate. The result showed a positive reaction. Furthermore, flow cytometry on cobacterium sp. surface antigen was performed using this antibody, the antibody showed binding to M. avium subsp. avium cells. To determine whether surface-bound SurfS1.2 or SurfS2.2 could influence mycobacterial growth, M. avium subsp. avium was grown in Middlebrook broth, the antibody prevented clumping in M. avium subsp. avium cells. The antibody was also used to magnetically separate M. avium subsp. paratuberculosis from faecal samples (Berger et al, 2006; pmid:16960114).
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