CloneID: MS-C2
Antigen Long Description: The original human IgG1 version of this antibody was obtained in the following manner: First, white matter tissue was dissected at autopsy from subjects with clinically defined MS, and individual B lineage cells were identified and captured. Multiplex PCR was used to amplify the Ig heavy and light chain variable regions, and recombinant IgGs were prepared from the Ig variable region sequences with IgG1 heavy chain and kappa constant domains.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P07196; Q9UN70
Specificity Statement: This antibody has been shown to bind to NF-L (neurofilament light) and protocadherin PCDHGC3.
Application Notes (Clone): The original human IgG1 version of the recombinant antibody MS-C2 was constructed in order to explore the antigen specificity of the Multiple Sclerosis B cell response (Willis et al, 2015; pmid: 26648933). The antibody was shown to bind two important neural antigens: neurofilament light (NF-L; strong binding with absorbance values that exceeded the mean +2SD of the control data set) and protocadherin PCDHGC3 (Willis et al, 2015; pmid: 26648933). Protocadherin gamma subfamily C, three (PCDHGC3) transcript variant three was of particular interest as a candidate autoantigen as protocadherin isoforms are predominantly expressed in the nervous system and have been implicated in human neurological disorders.
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