CloneID: CX-5
Antigen Long Description: The original rat IgG1 version of this antibody was raised by immunizing rats against mouse KLRK1.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original rat IgG1 format for improved compatibility with existing reagents assays and techniques.
Uniprot Accession No.: O54709
Specificity Statement: This antibody recognizes mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1 thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.
Application Notes (Clone): It has been shown that CX5 is a non-depleting antibody, which blocks binding of NKG2D to its ligands and mediates internalization of the receptor. This antibody has been widely used to block NKG2D (Wang et al., 2018; pmid: 29447423; Yang et al., 2017; pmid: 28455530) as well as to detect NKG2D-posotive cells via flow cytometry (Hosomi et al., 2017; pmid: 28747426).
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