CloneID: Nb43
Antigen Long Description: An adult male Bactrian camel was immunized subcutaneously with the purified Nsp4-NHis recombinant protein. A camel VHH library was then constructed and panned against Nsp4.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q9YN02
Specificity Statement: The antibody is specific for Nsp4.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by indirect ELISA analysis. MARC-145 cell lines expressing nanobodies were generated and infected with PRRSV strain SD16. At 24 h post-infection the antibody inhibited infectious virus release by about 99%. Further, the antibody protected MARC-145 cells from any virus-induced cytopathic effect and fully blocked PRRSV replication (Liu et al. 2016; PMID: 27010387).
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