CloneID: sHIgM46 (LYM46)
Antigen Long Description: The original antibody was isolated from the sera obtained from 102 patients with a wide variety of conditions including Waldenstrom’s macroglobulinemia, multiple myeloma, lymphoma, and monoclonal gammopathy.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Human IgM format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody recognizes and binds an epitope on the cell surface of human oligodendrocytes and central white matter of the cerebellum. This antibody also cross reacts with antigens present on mouse and rat oligodendrocytes.
Application Notes (Clone): This antibody is directed against the cell surface antigen of oligodendrocyte and promotes significant remyelination in a virus mediated model of multiple sclerosis (MS). A single 0.5 mg peripheral dose of either of two human IgMs (sHIgM22 and sHIgM46) promoted significant remyelination in chronically Theiler’s murine encephalomyelitis virus (TMEV) infected mice (PMID: 10841576). This antibody was used in the immunohistochemical staining of 8 week SJL/J mouse cerebellar slices and was found to bind the white matter the internal granular layer, Purkinje cell soma, and small cells within the molecular layer (PMID: 15198125). This antibody was also used in the immunohistochemical and immunocytochemical analysis of active MS lesions from 83 biopsies and autopsies of MS patients and was also used in the identification of four different pathologic subtypes of active MS lesions (PMID: 21961063)
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