CloneID: #9
Antigen Long Description: The original antibody was raised from intestinal B cells isolated from adults undergoing bariatric surgery for obesity.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q2VE61; P11193; A0A385LUU7; A0A4D6QBC5
Specificity Statement: This antibody is specific for VP8 of the rotavirus.
Application Notes (Clone): The original antibody was used for an ELISA on virus-like particles, bacterially expressed VP8 and VP5 (Nair et al., 2017; PMID:28637924). Further this antibody was used for a neutralization assay by using the antibody virus mixture on MA104 cells (Nair et al., 2017; PMID:28637924), and on MA104, HT-29, CaCo-2, CV-1, and HEK293 cells (Feng et al., 2019; PMID:31403468). This antibody was used for immunofluorescence on RV-infected MA104 cells (Nair et al., 2017; PMID:28637924). The structure of this antibody was determined using x-ray crystallography (Feng et al., 2019; PMID:31403468).
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