CloneID: PCT Nb2
Antigen Long Description: The original antibody was isolated by panning a camel-derived phage display nanobody library against PCT.
Origin Pub PMID: 25944262
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a chimeric antibody, developed from the original camelid VHH antibody, specifically engineered for enhanced compatibility with existing reagents, assays, and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The antibody is specific for PCT. PCT is a protein consisting of 116 amino acids. PCT is the peptide precursor of calcitonin, a hormone that is synthesized by the parafollicular C cells of the thyroid and involved in calcium homeostasis.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis (CN104404630A). The binding affinity of the VHH fragment to PCT was measured by surface plasmon resonance (Kd= 1.3 nM). The original format of the antibody was successfully used as a capture antibody, together with anti-PCT Nb3, for the development of a sandwich ELISA for the detection of PCT (Yan et al., 2015; PMID: 25944262).
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