CloneID: 29F.1A12
Antigen Long Description: The antibody was raised by immunizing rats with plasmid DNA containing PD-1.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q02242
Specificity Statement: This antibody recognizes an epitope on mouse PD-1.
Application Notes (Clone): This antibody was used for detection of PD-1 in CHO cells using flow cytometry. The ability of the antibody to block PD-1 interaction with PD-L1 or PD-L2 on CHO cells in an in vitro blocking assay was tested; the clone blocked PD-L1 Ig and PD-L2 Ig binding at high concentration, but only blocked 35% of PD-L1 Ig or PD-L2 Ig binding at 10 ug/ml. This antibody was also used for immunohistochemical studies of various mouse tissues, such as heart (Liang et al, 2003; pmid: 14515254). In vivo anti-tumor effect in terms of level of tumor growth reduction was studied, a significant tumor growth reduction was observed in the tumors from mice treated with the antibody (Tobias et al, 2020; pmid: 32528470). The antibody was used for the detection of PD-1 in EL4 cells using flow cytometry. The antibody was used for cross-blocking with the unconjugated version of the same clones and it was reported to prevent PD-1 from interacting with PD-L1 (Polesso et al, 2021; pmid: 33684223). The antibody was used as a positive control for CT26 tumor model, a murine model of colon cancer (Kaumaya ey al.; pmid:33117602).
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