CloneID: BT613
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing transgenic mice with PD-L1, followed by antibody library construction and phage display-based selection against PD-L1.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q9NZQ7
Specificity Statement: The original antibody is specific for PD-L1. The antibody recognises human and mouse PD-L1.
Application Notes (Clone): The binding affinity of the antibody to PD-L1 was measured by surface plasmon resonance. The binding affinity of the antibody was measured on the cell lines with high expression of PD-L1 by FACS. The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody had good activity in blocking the binding of PD-L1 to PD-1 and PD-L1 to B7-1 is shown by ELISA analysis. The antibody could block the binding of PD-1 and PD-L1 at the cellular level in in vitro experiments. The antibody was used for in vivo experiments on colon cancer tumor model. B-hPD-1 mice with human PD-1 and MC38-hPD-L1 colon cancer cells with human PD-L1 were treated with the antibody. Results showed inhibition of tumor volume growth in mice treated with the antibody.
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