CloneID: PLA 2-mc8
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with natural phospholipase A2 protein purified from crude venom of Trimeresurus stejnegeri.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P82896
Specificity Statement: This antibody reacts specifically with phospholipase A2 (PLA2) protein present in the venom of Trimeresurus stejnegeri. PLA2 inhibits platelet aggregation induced by ADP, arachidonic acid and PAF. It acts in an enzymatic independent manner on a proteinase-activated receptor (PAR1, F2R) to evoke calcium release through the inositol 1,4,5-trisphosphate receptor (ITPR1, IP3R) and induces mouse aorta contraction.
Application Notes (Clone): This antibody can be used for the detection of PLA2 protein of Trimeresurus stejnegeri by ELISA. The binding specifcity of the antibody was further confirmed by detection of the protein in a western blot (CN113930408; PMID: 34899734).
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