CloneID: mAb 2A4
Antigen Long Description: The original antibody was generated by immunizing BALB/C mice with purified human podocalyxin-glutathione S-transferase (GST) fusion protein containing the intracellular, transmembrane, and a part of the extracellular domain of human PCLP.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: O00592
Specificity Statement: The antibody is specific for human podocalyxin. The epitope recognized by the antibody is located on the intracellular domain of podocalyxin. Podocalyxin, a sialoglycoprotein, is thought to be the major constituent of the glycocalyx of podocytes in the glomerulus (Bowman's capsule) in the kidneys. It is a member of the CD34 family of transmembrane sialomucins.
Application Notes (Clone): The antibody recognized rabbit PCLP1 in human renal cortical sections on immunofluorescence. The antibody detected PCLP by western blot analysis. The antibody recognized and immunoprecipitated rat podocalyxin (Kershaw et al., 1997; PMID: 9188463). The antibody detected PCLP in HEV of tonsil by immunofluorescence analysis. The antibody stained HEV of paraffin-embedded samples of lymph node and appendix by immunohistochemistry (Sassetti et al., 1998; PMID: 9625756).
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