CloneID: 22-19
Antigen Long Description: The original antibody was generated by immunizing mice with semipurified fraction of potato virus Y (PVY) as antigen.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P22602
Specificity Statement: This antibody bind the nuclear inclusion A (NIa) protein of the Potato virus Y (PVY). Potato virus Y is a monopartite, single stranded RNA virus, plant pathogen that infects mainly Solanaceous plants including, potato, tomato, pepper, tobacco and eggplant. There are multiple strains of the virus including the common strain, PVYO, which causes mosaic symptoms in most hosts. The necrotic strains PVYN and PVYNTN both cause a veinal necrosis on tobacco; PVYNTN also causes a tuber necrosis in certain potato varieties. This necrosis is referred to as potato tubers necrotic ringspot disease (PTNRD). In general, the necrotic strains tend to cause a more mild mosaic symptom than PVYO in potato. PVY is transmitted in a non-persistent manner by aphids.
Application Notes (Clone): This antibody was used for in situ localization of potato virus Y NIa protein using immunofluorescence staining. The staining showed the accumulation of the NIa protein in both nucleus and cytoplasm. The binding characterization of the antibody was done using ELISA (PMID: 16884787). In vivo, this antibody was able to detect different forms of the NIa protein including precursors and cleavage products and also able to inhibit the cleavage of the polyprotein detected in the semipurified NI (PMID: 11556707).
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