CloneID: DS7
Antigen Long Description: The original antibody was isolated from the phage display library generated from the bone marrow tissue of 12 donors. The screening of the library was done against recombinant hMPV F protein.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q6WB98
Specificity Statement: This antibody recognizes and binds the Human metapneumovirus F protein residues located in the DI and DII head domains. This antibody binds MPV F in its pre-fusion conformation and does not specifically bind to MPV F in its postfusion conformation. Human metapneumovirus (hMPV) is a member of the Paramyxoviridae family/Pneumovirinae subfamily and shares many common features with respiratory syncytial virus (RSV), another member of the same subfamily. hMPV causes respiratory tract illnesses that, similar to human RSV, occur predominantly during the winter months and have symptoms that range from mild to severe cough, bronchiolitis, and pneumonia. It cross-reacts with lineage A1, A2, B1.
Application Notes (Clone): The binding characterization of this antibody to hMPV F protein was done using ELISA. The binding of the antibody was also confirmed using immunofluorescent assays of LLC-MK2 cell culture monolayers were infected with hMPV. The fab version of this antibody neutralized the parent strain of hMPV with a 60% plaque reduction activity of 1.1 μg/ml and bound to hMPV F with an affinity of 9.8 ×10^(−10) M. This antibody was reported to reduce viral titers in the lungs of cotton rats when given therapeutically and modestly reduced titers in nasal tissues. There was a dose-response relationship between the dose of DS7 and viral titers. This antibody was capable of neutralizing strains from the A1 and B1 lineages in addition to the A2 lineage but failed to neutralize the B2 strain (PMID: 17522220).
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