Anti-Protein C (IgG1-7)

Anti-Protein C [IgG1-7], Recombinant, IgG1 kappa, Mouse

CloneID: IgG1-7

Antigen Long Description: The original antibody was generated from the original mouse antibody HPC-4 by performing site directed mutagenesis, consensus and resurfacing methods.

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Uniprot Accession No.: P04070

Specificity Statement: This antibody specifically recognizes and binds the ProC epitope tag comprising the amino acid sequence 'EDQVDPRLIDGK'. This antibody binds the zymogen form of human Protein C in the activation peptide (spanning the thrombin cleavage site) and blocks protein C activation by thrombin–thrombomodulin both in vitro and in vivo. It does not react with activated protein C.

Application Notes (Clone): This antibody is the humanized version of anti-Protein C antibody HPC-4. The binding of this antibody to protein C is calcium dependent. This humanized anti-protein C antibody exhibited calcium dependent binding which was significantly increased over that of the murine antibody HPC-4. The binding characterization of this antibody was performed using ELISA (PMID: 9680194). This antibody can be used to purify protein tagged with this 'EDQVDPRLIDGK' epitope.

• SKU: ABAAb03473-1.1-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03473-1.1-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Recombinant
• Application: ELISA, Blocking
• Isotype: IgG1 kappa
• Host: Mouse