CloneID: RNL-3
Antigen Long Description: The original antibody was generated by immunizing a BALB/c mouse with the small cell lung cancer cell line NCI-H82.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q16799
Specificity Statement: The antibody binds Reticulon-1A/B / NSP-A/B. The antibody recognizes an epitope located within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B).
Application Notes (Clone): The antibody detected NSP-A and B from NCI-H82 cells by western blot analysis. Immunofluorescence using the antibody revealed NSPs localized in the endoplasmic reticulum (ER) of NCI-H524 cells and migrated to the ER of COS-1 cells after transfection with the NSP-B construct. The antibody recognized a sub-set of neuro-endocrine tissues and tumours by immunohystochemistry. In normal tissues, the antibody reacted with brain Purkinje cells, pancreatic islet cells, some cells in the pituitary gland and some (peripheral) nerve fibres. Further, spleen reticular fibres, prostatic epithelium and some suprabasal cells of the tongue epithelium were also positive with the antibody. In the lung, positive staining was observed in the (sero)-mucous glands. While in non-pulmonary human tumours, a staining reaction was observed in a sub-set of neuro-endocrine tumours, such as pituitary adenomas and insulinomas. Moreover, some other malignancies, such as medullary Schwannoma, prostate carcinomas, thyroid carcinomas and melanomas, were positive (Senden et al., 1994; PMID: 7515034).
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