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Application: Useful for binding assays.
Assay Conditions: A 96-well plate was coated with RNF43, Avi-Tag, His-Tag Recombinant (#102360) (100 ng/well in PBS) at 4oC overnight. The plate was washed three times with PBST, and 200 µl Blocking Buffer 3 was added for 2 hours at room temperature (RT). After three washes in PBST, a 1:2 serial dilution of Anti-RNF43, Avi-Tag, Biotin-Labeled Antibody (#102549) was prepared in PP-02 Assay Buffer (#82620), was added and incubated at RT for 1 hour. The plate was washed three times and incubated with Anti-human Fc-HRP conjugate diluted of 1:1000 in Blocking Buffer 3 for 1 hour at RT. After three washes, 100 µl of the ELISA ECL Substrate (#79670) was added to each well and chemiluminescence was measured.
Background: RNF43, or ring finger protein 43, is an E3 ubiquitin ligase that acts a tumor suppressor to negatively regulate the WNT ((wingless-related integration site) signaling pathway through promoting the degradation of frizzled WNT receptors. Loss of function mutations in RNF43 have been shown to be a predictive biomarker in favorable response to anti-BRAF ((B-Raf proto-oncogene serine/threonine kinase)/EGFR ((epidermal growth factor receptor) therapies in colorectal cancer patients. RNF43 mutations are frequent predictors, and therefore of high clinical interest, in pancreatic, gastric, and endometrial cancers. Further studies will reveal more on the cancer signaling pathways and effective combinatorial therapies involving RNF43.
Description: This anti-RNF43 antibody is a purified, humanized recombinant monoclonal antibody that recognizes human RNF43 (ring finger protein 43). The heavy chain contains a C-terminal Avi-tag™. This antibody has been tested for binding activity in an ELISA assay.
Endotoxin Level: 10.2 EU/mg
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: Avi-tag
Target: RNF43
Uniprot: Q68DV7
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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