Anti-S protein (RBD) (1E2)

Anti-S protein (RBD) [1E2], Recombinant, IgG-Fc Fusion, Rabbit

CloneID: 1E2

Antigen Long Description: The original antibody was generated from a panel of humanized single domain antibodies derived from a synthetic library. The antibody was isolated by panning against SARS-CoV-2 spike receptor-binding domain (RBD).

Buffer Composition: PBS only.

Uniprot Accession No.: P0DTC2

Specificity Statement: The antibody binds to the RBD of the Spike protein of SARS-CoV.

Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding affinity of the VHH fragment to SARS-CoV-2 RBD was measured by surface plasmon resonance (Kd= 35.5 nM). The VHH showed half maximal neutralization concentration (EC50) of 0.069 µg/mL and 0.2401 µg/mL against SARS-CoV-2 pseudotypes, and authentic SARS-CoV-2, respectively. Fusion of the human IgG1 Fc to the VHH improved the neutralizing activity compared to the original format of the antibody. The Fc-fused VHH demonstrated potency with EC50 of 0.538 nM. (Chi et al, 2020; pmid: 32913273)

• SKU: ABAAb03638-23.159-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03638-23.159-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Virus
• Clonality: Recombinant
• Application: ELISA, Neutralization, Super-Resolution Microscopy
• Isotype: IgG-Fc Fusion
• Host: Rabbit