CloneID: 5F8
Antigen Long Description: The original antibody was generated by using phage display technology by biopanning a full synthetic, humanized phage display library with recombinant RBD protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P0DTC2
Specificity Statement: The antibody binds the receptor binding domain (RBD) of the SARS-CoV-2 Spike protein.
Application Notes (Clone): The binding affinity of the VHH fragment towards SARS-CoV-2 or SARC-CoV RBD was measured using surface plasmon resonance (Kd= 0.996 and 239,2 nM respectively). The neutralization activity of the VHH fragment with SARS-CoV-2 pseudotypes or SARS-CoV-2 was tested, the showing half maximal neutralization concentration (EC50) of 0.0009 µg/mL and 0.51 µg/mL respectively. Competition-binding assay showed the clone could just partially prevent binding of SARS-CoV-2 RBD to ACE2. Neutralization assay of the bivalent form of the clone fused with human IgG1 Fc against SARS-CoV-2pp was performed, showing EC50= 0.004 µg/mL (Chi et al, 2020; pmid: 32913273).
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