CloneID: 1H3-1
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with SN38-BSA conjugate.
Buffer Composition: PBS only.
Specificity Statement: This antibody binds SN-38, which is an active metabolite of irinotecan (a semisynthetic analog of camptothecin - a topoisomerase I inhibitor) generated by carboxyl esterases through hydrolysis. SN-38 has potential antineoplastic activity. It binds and inhibits topoisomerase I by stabilizing the cleavable complex between topoisomerase I and DNA, resulting in DNA breaks, inhibition of DNA replication, and apoptosis. In vitro cytotoxicity assays have shown that SN-38 is 1,000-times more cytotoxic than irinotecan against various cancerous cells. SN-38 is commonly used as a cytotoxic drug payload in the ADC field.
Application Notes (Clone): The binding reactivity of this antibody to SN38-BSA conjugate was determined using ELISA. The binding affinity of this antibody to SN38-KLH conjugate was determined using surface plasmon resonance (SPR). It was reported that this antibody binds SN38-KLH conjugate with a binding affinity of Kd=3.06 nM (CN115197323).
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