CloneID: MABaP1
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizng BALB/c mice with 10 mg BaP1 obtained from B. asper venom emulsified in incomplete Marcol/Montanide adjuvant.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P83512
Specificity Statement: This antibody reacts with snake venom metalloproteinase (SVMP) BaP1 present in the venom of Bothrops asper. BaP1 is a P-I class of SVMP relevant in the local tissue damage associated with envenomations by Bothrops asper. This metalloproteinase exhibits a weak hemorrhagic activity and rapidly degrades the Aalpha-chain (FGA) of fibrinogen, and later on, degrades the Bbeta-chain (FGB) of fibrinogen.
Application Notes (Clone): The antibody can bind snake venom metalloproteinases BaP1, whole venom of B. asper and BnP1 (B. neuwiedi) in an ELISA. This antibody completely neutralized in vivo hemorrhage, muscle necrosis, and inflammation induced by the toxin. This antibody completely neutralize myotoxicity induced by BaP1. In general it was capable of neutralizing biological effects of this SVMP (PMID: 20674587; 20674587).
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