CloneID: MT251
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with recombinant human TIGIT protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q495A1
Specificity Statement: The original antibody is specific for TIGIT. The antibody does not cross-react with LAG-3, PD-1 and PD-L1.
Application Notes (Clone): The original mouse antibody was subjected to humanized modification, keeping a similar binding activity. The specificity of the original and the humanized format of the antibody was confirmed by ELISA analysis (EC50= 0.09901 μg/mL and 0.07412 μg/mL respectively). The original format of antibody blocked the binding activity of TIGIT and CD155. The original format and the humanized version of the antibody could recognize the TIGIT antigen expressed by Jurkat/TIGIT cells by FACS analysis. The original format of the antibody was able to effectively block the binding of TIGIT to CD155 (CN112794909).
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